Topology of asialoglycoprotein receptor in rat liver subcellular fractions: A ferritin immunoelectron microscopic study.
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چکیده
منابع مشابه
Ferritin immunoelectron microscopic localization of 5'-nucleotidase on rat liver cell surface
Rat livers were prefixed by perfusion with 0.6% glutaraldehyde and briefly homogenized with a Teflon-glass homogenizer. The prefixed cells isolated by low-speed centrifugation in high yield effectively preserved the original polygonal shape and polarity. These cells were incubated with ferritin-antibody conjugates monospecific for rat liver 5'-nucleotidase, and the localization of the enzymes o...
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1. Rat-liver mitochondria showed a decrease in amino acid production after preparation in 0-25m-sucrose containing EDTA (lnm), but an increase in water content. When EDTA was replaced by Mn2+ (Imm) or succinate (lmM), both amino acid production and water content were lowered, whereas preparation in 0-9% potassium chloride caused an increase in both. 2. Amino acid production by rat-liver homogen...
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The localization of various metabolic processes of cells in particulate and soluble intracellular components has been studied intensively (l-3). The effect of added metals on these processes has also received attention (4-l3), but information on the metal content of the cell fractions is limited (14-16). The association of metals with proteins has been divided into two categories on the basis o...
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I. Black-hooded weanling rats were given a copper-deficient diet or diets providing 3 ppm Cu with or without supplements containing combinations of molybdate, sulphate and sulphide salts to provide 35 ppm molybdenum and z pg atoms sulphurlg. Changes in weight and blood haemoglobin concentration were studied during 48 d of treatment. The subcellular distribution of Cu and Mo in the liver was sub...
متن کاملElectron Microscopic Examination of Subcellular Fractions
Large granule fractions, containing about 80% of the cytochrome oxidase of the tissue, were isolated from rat liver and used to prepare thin pellicles of packed particles which were submitted to quantitative electron microscopic examination. Various parameters describing the mitochondrial population were determined by measuring the size and number of mitochondrial profiles in sections, and the ...
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ژورنال
عنوان ژورنال: Cell Structure and Function
سال: 1984
ISSN: 0386-7196,1347-3700
DOI: 10.1247/csf.9.391